Manganese import protects: Salmonella enterica serovar Typhimurium against nitrosative stress

S Yousuf; JE Karlinsey; SL Neville; CA McDevitt; SJ Libby; FC Fang; ER Frawley

Journal article

Manganese import protects: Salmonella enterica serovar Typhimurium against nitrosative stress

S Yousuf, JE Karlinsey, SL Neville, CA McDevitt, SJ Libby, FC Fang, ER Frawley

Metallomics | ROYAL SOC CHEMISTRY | Published : 2020

DOI: 10.1039/d0mt00178c

Download PDF

Abstract

Nitric oxide (NO) is a radical molecule produced by mammalian phagocytic cells as part of the innate immune response to bacterial pathogens. It exerts its antimicrobial activity in part by impairing the function of metalloproteins, particularly those containing iron and zinc cofactors. The pathogenic Gram-negative bacterium Salmonella enterica serovar typhimurium undergoes dynamic changes in its cellular content of the four most common metal cofactors following exposure to NO stress. Zinc, iron and magnesium all decrease in response to NO while cellular manganese increases significantly. Manganese acquisition is driven primarily by increased expression of the mntH and sitABCD transporters fo..

View full abstract

University of Melbourne Researchers

Stephanie Neville Author

Christopher McDevitt Author

Related Projects (4)

NOVEL INSIGHTS INTO THE MOLECULAR MECHANISMS OF MANGANESE RECOGNITION AND ACQUISITION BY PATHOGENIC BACTERIA

Streptococcus pneumoniae is the world’s foremost bacterial pathogen. In Australia, bacterial infections are responsible for more than 9000 d..

INVESTIGATING THE ANTIMICROBIAL ACTIVITY OF ZINC AT THE HOST-PNEUMOCOCCAL INTERFACE

Streptococcus pneumoniae is a human-only bacterium that is responsible for killing more than one million people every year. This project wil..

NEW MOLECULAR TOOLS TO STUDY THE MECHANISMS OF BACTERIAL METAL HOMEOSTASIS

This project aims to provide new insight into how metal ion uptake is regulated. It will precisely measure the cellular concentrations of me..

MOLECULAR BASIS OF ZINC ACQUISITION BY STREPTOCOCCUS PNEUMONIAE

Streptococcus pneumoniae is the world’s foremost bacterial pathogen. In Australia, bacterial infections are responsible for more than 9000 d..

Grants

Awarded by Rhodes College

Funding Acknowledgements

We thank Ms. Sarah Hasty for technical assistance with ICP-MS sample preparation. S. Y. and E. R. F. were supported by startup funding from Rhodes College. J. E. K., S. J. L. and F. C. F. were supported by NIH AI112640 and AI118962. S. L. N. and C. A. M. were supported by NHMRC 1140554 and 1180826. S. L. N. was supported by NHMRC Early Career Research Fellowship 1142695. C. A. M. was supported by Australian Research Council Future Fellowship FT170100006.